Isolation and characterization of pentachlorophenol degrading bacteria from industrial wastes
1999
Ali, S.S. | Samad, Z. | Shakoori, A.R. (Punjab Univ., Lahore (Pakistan). Dept. of Zoology)
Effluent samples collected from tanneries of Kasur and paper industry Lahore were spread on agar plates containing glucose deficient M9 media in which the pentachlorophenol (PCP) was added as the only carbon source. Four bacterial strains CB17 to CB20 were isolated and on the basis of physical and biochemical characterization identified as belong to genus Listeria sp., Lactobacillus sp., Pseudomonas sp. and Xanthomonas. sp., respectively. The pure cultures of these isolates were prepared and their tolerance for PCP was determined upto 12 microgram/ml. The minimum inhibitory concentration (MIC) for CB17 was 10 microgram/ml, for CB18 it was 11 microgram/ml, for CB19 and CB20 it was 12 microgram/ml. Optimum growth conditions of these isolates were determined. The optimum temperature for the growth of all these isolates was 37 degree centigrade. The optimum pH for CB17 strain was 6.0, for CB18 and CB19, 5.5 and for CB20 it was 7.0. Growth curves for these isolates were prepared: They showed log and stationary phases. The span of log phase was 8, 8, 12 and 24 hours in 4 strains, respectively. The bacterial isolates were also tested for resistance against some heavy metals i.e., Cu+2, Cr+6 and Pb+2. The MIC for Cu+2 was 175, 350, 400 and 275 microgram/ml, for Pb+2 it was 100, 200, 500 and 250 microgram/ml and for Cr+6 200, 275, 175 and 225 microgram/ml respectively for these strains. All isolates showed resistance to antibiotics such as urixin, erythromycin, cefazolin and SXT. The MICs of these isolates against PCP proved that these insecticide degrading bacteria although, can be used for the purpose of environmental cleanup but they showed relatively, moderate potential for degradation of PCP.
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