Serum retinol level of human subjects fed carotene rich diet
2007
Saleemullah (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Agricultural Chemistry) | Ahmad, M.N. (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Agricultural Chemistry) | Shah, H.U. (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Agricultural Chemistry) | Khalil, I.A. | Suleman, M.
The study was conducted in 2005 at Department of Agricultural Chemistry, NWFP Agricultural University, Peshawar, Pakistan. The objective was to observe the apparent effect of (beta-carotene on serum retinol level of 10 volunteers who were fed spinach as a source of (beta-carotene. The vitamin A contents before and after feeding the test diet was determined by HPLC (High Performance Liquid Chromatography). The data revealed that average vitamin A content of the sera varied from 10.7 to 35.0 mug/dl before feeding the test diet. After feeding the test diet, the average Serum retinol content was increased from 20.07 to 37.1 mug/dl in majority of the subjects. Considerable variation was noted among the subjects in response to the same dose of carotenous diet. In four subjects, there was marked increase in the sera retinol content, while in five subjects, though there was an increase but it was not pronounced. One of the subjects showed no response to conversion of beta carotene to vitamin A. It was worth mentioning that no subject complained of the adverse effects of the diet. The subjects remained healthy during the experimental period and afterwards. It was therefore, concluded that carotene had no side effects on human health, rather the diet was protective and healthy as a source of vitamin-A.
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