Over-expression of GmWRKY111 enhances NaCl tolerance of salt-sensitive genotype of Glycine max
Xu, Z.L. (Nanjing Agriculture Univ., Nanjing (China). Coll. of Agriculture) | Ali, Z. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics) | Yi, J.X. (Jiangsu Academy of Agricultural Sciences, Nanjing (China). Inst. of Biotechnology) | Xu, L. | Liu, X.Q. | He, X. | Huang, Y.H. | Karim, I. | Rasul, M. | Ma, H.X. | Zhang, D.Y.
WRKY transcription factors are a large family in higher plants, play significant functions in plant growth and stress response, such as plant development, metabolism, and responses to biotic and abiotic stresses. In this study, we identified one WRKY transcription factor gene GmWRKY111 from soybean with an open reading frame of 1197 bp encoding 398 amino acids with a molecular weight of 44 kD and a pI of 5.7. Analysis of expression profiles GmWKRY111 highly expressed in root at differential growth period, and weakly expressed in flower, stem and leaf, and significantly responded to 200 mM NaCl. In addition, the multiple alignments analysis indicated that GmWRKY111 possessed a conserved WRKYGQK domain and a zinc-finger structure; the phylogenetic tree analysis showed that GmWRKY111 belongs to group IIc clade. Meanwhile, Over-expression of the GmWRKY111 improved seedling tolerance to salt in composite transgenic soybean plants. These results indicate that GmWRKY111 improves the ability of plant to confer salt tolerance. Further studies are needed to explore its interaction with other proteins and explore the pathway in which it is involved.
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