Industrial enzyme production by Mucor geophillus IBGE 05
Ahmed, K. (N.E.D. Univ. of Engineering and Technology, Karachi (Pakistan). Dept. of Chemistry) | Valeem, E.E. (Karachi Univ. (Pakistan). Inst. of Marine Science) | Qamar-ul-Haq (Federal Urdu Univ. of Arts, Sciences and Technology, Karachi (Pakistan))
Optimization parameters in submerged fermentation were studied for the production of alpha-amylase from Mucor geophillus IBGE 05 using agricultural wastes (sunflower waste, cotton stalk and rice husk) as well as agro industrial wastes (date syrup and molasses) as sources of carbon. Optimal conditions for the production of alpha-amylase (4.87 U/mL) by Mucor geophillus IBGE 05 were observed when the strain was grown on culture medium M1 containing yeast extract as a source of nitrogen, molasses as a source of carbon after 48 h of incubation at 35 degree C, initial pH 6.5, inoculum size of 5x10(6) conidia in 50 mL of culture medium and agitation rate of 150 rpm. The strain was thermostable (up to 60 degree C) and pH (up to 9.0) so it has a potential to be used in industries for enzyme production.
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