Possibility of applying feruloyl esterase from Aspergillus niger A.n.8 for degradation of a cell wall complex in selected cereals
Trzcinska, M.E-mail:[email protected] | Halasinska, A.G. | Wetoszka, U. | Sieliwanowicz, B.(Institute of Agricultural and Food Biotechnology, Warsaw (Poland). Dept. of Technical Microbiology and Biochemistry)
A preparation of feruloyl esterase was obtained from post culture medium of Aspergillus niger A.n.8. 5-day culture. The preparation demonstrated the maximal activity at pH 4.3 and a temperature of 55 deg C, molecular weight of the investigated enzyme was 30 000 Da. The preparation released ferulic acid from natural cereal arabinoxylanes. Synergy between the activity of the preparation obtained and xylanase activity was proved. The addition of the feruloyl esterase preparation significantly increased the amount of reducing saccharides, released by xylanase from a substrates containing natural cereal arabinoxylanes
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