Evaluation of the biodiversity of root nodule bacteria isolated from Calliandra Calothyrsus Meisn. trees growing in Central America, Cameroon, Kenya and New Caledonia
2003
Dresler-Nurmi, A. | Räsänen, L.A. | Lindström, K.
Semi-automated restriction fragment length polymorphism (RFLP) of 16S rDNA was used to evaluate the biodiversity of 293 strains of bacteria collected from root nodules of Calliandra calothyrsus in Central America, Africa and New Caledonia. Thirty representatives of defined Rhizobium, Agrobacterium, Sinorhizobium, Mesorhizobium and Bradyrhizobium species were included in the study. Major genotypes: A, B, C, D, E and F with 116, 63, 40, 24, 14 and 12 strains and minor genotypes: G, H, I, J, K, L and M with 8, 6, 6, 1, 1, 1 and 1 strains, respectively were used to construct the UPGMA dendrogram. Genotypes A, C E, G, H, J and 1 were clustered with established rhizobial species and genotypes B, D, F remained unclustered. Representatives of genotype A were initially clustered with R. tropici while Bradyrhizobium-like isolates represented mainly genotype B. Inoculant strains used in field tests (CCNC26, CCCR15, CCCR1, CCC22, KWN35, KCC6) represented genotypes A, C and D. The biodiversity of isolates from Central America and New Caledonia was greater than that of isolates collected in Kenya and Cameroon. (Résumé d'auteur)
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