Relationship between Kappa-Casein gene polymorphism with milk economic traits in native cattle of Guilan province
2017
Nazemi, F. | Mirhosseini, S. Z. | Ghavi Hossein-Zadeh, N. | Dehghanzadeh, H. | Mehdizadeh, M.
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Show more [+] Less [-]In this research, the relationship between polymorphism of CSN (Kappa-Casein) gene and production traits in Guilan native cattle was studied. Blood samples were taken from 100 cows Fouman, Rasht and Astaneh-ye-Ashrafiyeh. Genomic DNA was extracted by modified salting out method. A 350 bp fragment of the bovine kappa-CSN gene was amplified by Polymerase Chain Reaction (PCR) using a specific primer. The PCR products were digested by Hinf I to determine kappa-casein genotypes with PCR-RFLP marker. Observed frequencies for genotypes AA, AB, BB were 0.48, 0.52, 0.00 and allelic frequencies for A and B were 0.74 and 0.26, respectively. The chi-square test results showed that the studied population were not at Hardy-Weinberg equilibrium. Comparison of least square means indicated that the polymorphism has a significant effect on milk yield, fat and protein percentage (P<0.01). The cows with genotype AA produced 0.81 kg more milk over the cows with genotype AB. The cows with genotype AB produced 0.1 and 0.14 percentages higher milk fat content compare to the cattle with genotypes of AA (P<0.01). The results also revealed that the cattle with genotypes of AA produced milk with lowest fat and protein content.
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