Enzymatic and mRNA Transcript Response of Ovine 6-Phosphogluconate Dehydrogenase (6PGD) in Respect to Different Milk Yield
2010
Trivizaki, Stamatina(Department of Animal Science, Laboratory of Animal Breeding and Husbandry) | Laliotis, George P.(Department of Animal Science, Laboratory of Animal Breeding and Husbandry) | Bizelis, Iosif(Department of Animal Science, Laboratory of Animal Breeding and Husbandry) | Charismiadou, Maria A.(Department of Animal Science, Laboratory of Animal Breeding and Husbandry) | Rogdakis, Emmanuel(Department of Animal Science, Laboratory of Animal Breeding and Husbandry)
Ovine 6-phosphogluconate dehydrogenase (6PGD) is an enzyme of the pentose phosphate pathway, providing the necessary compounds of NADPH for the synthesis of fatty acids. Much of research has been conducted both on enzymatic level and on molecular level. However, to our knowledge, any correlation between enzymatic activity and 6PGD gene expression pattern related to different physiological stages has not been yet reported. With this report, we tried to highlight if any correlation between enzymatic activity and expression of ovine 6PGD gene exists, in respect to different milk yield. According to the determined enzymatic activities and adipocytes characteristics, ewes with low milk production possessed a greater (P≤.001) 6PGD activity and larger adipocytes than the highly productive ewes. Although 6PGD expression pattern was higher in low milk yield ewes than in ewes with high milk production, this difference was not found statistically significant. Thus, 6PGD gene expression pattern was not followed by so rapid and great/sizeable changes as it was observed for its respective enzymatic activity, suggesting that other mechanisms such as post translation regulation may be involved in the regulation of the respective gene.
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