Evaluacion de resistencia a fusariosis en campo mediante inoculacion artificial.
1989
Garcia C.B.
Fusarium head scab is a serious disease in the southeastern part of the Buenos Aires Province, where in critical years it can cause wheat yield losses of more than 7 %. Fusarium epidemics were responsible for the disappearance of durum wheat from the region, which had been the major wheat-producing area in the country. Considering the importance of the disease and the instability of testing genetic materials in the field, artificial inoculation is necessary. Two methods of artificial inoculation, injecting inoculum into the spike and spraying inoculum at anthesis, were compared. The inoculum was a mixture of several monosporic strains which had been multiplied under optimal laboratory conditions. Inoculation was done in the field and spikes were covered to maintain moisture. Grain infection was assessed after threshing the grain in the laboratory, using trays containing moist cotton covered with filter paper. Also, the longitudinal disease development planes from the point of infection to the ends of the spike were developed. The injection methods produced infection more effectively than the simpler spraying method. The most advanced materials from the breeding program were tested using the injection method and a list of the top lines is included.
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