Effects of hepatic lipid accumulation on gluconeogenesis from glycerol in growing chicks.
Ohtani S. | Tanaka K.
All avian species maintained a high plasma glucose concentration under all physiological conditions. However, hypoglycemia was observed in the chicks affected by fatty liver and kidney syndrome, and it was suggested that excessive hepatic lipid accumulation depressed gluconeogenesis in liver. Therefore, such chicks could not maintain their plasma glucose concentration at a normal level during starvation for a long period. Glycerol derived from triacyglycerol during starvation is one of the major gluconeogenic substrates. In this experiment, the effects of excessive lipid accumulation in the liver on gluconeogenesis from glycerol were investigated with fed or fasted experimental fatty liver chicks. White Leghorn male chicks were reared under the standard management practice until they reached the age of 28 days, and they were divided into two groups. The first group served as control wherein the chicks were given commercial feed, while the second group were given the same feed added with 0.08% dienesterol diacetate and 0.025% propylthiouracil for 12 days (fatty liver chicks). Thereafter, chicks of each group were subdivided into three subgroups, respectively. Blood of chicks of the one subgroup immediately and of the other two subgroups after starvation for 24 or 72 hours, was sampled from the wing vein and injected with glycerol (2.4mg/100g body weight), blood samples were also obtained at 0.5, 1, 3 and 5 hours after glycerol injection. The concentrations of glucose and free fatty acids in plasma and glycerol i whole blood were determined. After the blood sampling, chicks were killed and the liver tissues were for determination of triacylglycerol contents.Show more [+] Less [-]