Produccion de anticuerpos monoclonales con aislamiento seleccionados del virus del mosaico dorado del frijol.
1994
Cancino M. | Abouzid A.M. | Morales F.J. | Purcifull D.E. | Hiebert E.
Two monoclonal antibodies prepared to purified virions of bean golden mosaic virus (BGMV) isolates from Guatemala (GA) and Dominican Republic (DR) and one monoclonal prepared to the coat protein of a BGMV isolate from Brazil (-BZ) expressed in E. coli., were identified as being useful serological probes on the basis of their differential reactivity to various geminiviruses. They were tested against the following geminiviruses: abutilon mosaic, BGMV isolates from Puerto Rico (-PR), and Homestead, Florida (-H), BGMV-BZ, -DR, and -GA isolates, euphorbia mosaic, rhynchosia mosaic, squash leaf curl, soybean yellow mosaic, an isolate from macroptilium in Florida (BGMV-FL), a new virus infecting cabbage (Brassicassae) in Florida (CGV), and tomato mottle. One monoclonal antibody reacted efficiently in Western blot and ELISA with all tested geminiviruses with the exception of CGV, indicating this monoclonal antibody has a broad spectrum of reactivity. Another monoclonal antibody reacted only with BGMV-DR, -GA, and -PR isolates. A third monoclonal reacted only with BGMV-BZ.
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