Race and biovar determination of Ralstonia solanacearum in the north west of Pakistan
2018
Junaid, Muhammad | Ahmad, Musharaf | Saifullah
Multiple comprehensive surveys were conducted during 2012, in order to know the current status of bacterialwilt (BW) of tomato caused by Ralstonia solanacearum (R. solanacearum), in the commercial tomato growingdistricts of Khyber Pakhtunkhwa (KP), Pakistan. A total of 74 locations covering all the 26 districts of the 7divisions of KP were visited for the presence of tomato plants showing BW symptoms. According to Polymerasechain reaction (PCR), the expected 281bp band was amplified from 25 candidates of R. solanacearum isolates,thus genetically confirming them to be R. solanacearum. These R. solanacearum isolates were subjected to raceidentification and biovar determination tests. Race differentiation was done using hypersensitive response (HR)test on tobacco plants; whereas 10% solutions of six different sugars including disaccharides (i.e. sucrose, lactoseand maltose), and sugar alcohols (i.e. manitol, sorbitol and dulcitol) in Triphenyl tetrazolium chloride (TTC)medium, were used for biovars determination. Results indicated that all PCR-confirmed R. solanacearum isolatesbelonged to race-1 and biovar-3. However, two isolates i.e. (R. solanacearum11-DIK1 and R. solanacearum10-MDN2) were found to belong to race-3, biovar-2.
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