Evaluation of the effectiveness of disinfectants against exogenous forms of cryptosporidia

Introduction. Cryptosporidiosis is a widespread protozoal disease of many animal species, as well as humans. The list of drugs used to destroy exogenous forms of oocysts of Criptosporidium parvum is limited, since the oocysts of the pathogen are difficult to destroy in the external environment due to their small size, the presence of a thick shell that protects them from adverse environmental factors, which makes them highly resistant to disinfectants. The purpose of the work was to analyze the effectiveness of preparations “Ligrocid” and “Virocid” for disinfection against Criptosporidium parvum oocysts. Materials and methods. A comparative assessement of the effectiveness of “Ligrocid” and “Virocid” at a concentration of 0.25% with an exposure time of 1 hour for disinfection against Criptosporidium parvum oocysts was carried out. Scrapings were taken from environmental objects (floor, walls, feeders, drinkers) using a brush for material sampling. Material for study was taken before treatment and a day after disinvasion. Native smears were prepared from the obtained samples, stained according to Cyl-Nielsen and microscoped at a magnification of 1000 times. The obtained results were statistically analyzed using the McNemar chi-square criterion. Research results, discussion. When examining smears taken before disinvasion, it was found that all surfaces were contaminated with Cryptosporidium oocysts. After disinvasion with “Ligrocide”, oocysts were detected only in three samples, and when using“Virocide” in five samples. Conclusion. Thus, the results of the study showed that “Ligrocid” for the disinvasion of exogenous forms of Criptosporidium parvum was 1.7 times (p<0,001) more effective than “Virocid”. It should be noted that the preparation “Ligrocid” is almost 10 times cheaper than “Virocid”.