Comparative titration test of sheep pox virus produced by Borrel method on sheep and sheep kidney tissue culture. | Borrel Metodu ile Üretilen Koyun Çiçek Virusunun Koyunlarda ve Doku Kültürlerinde Mukayeseli Olarak Titrasyon Testlerinin Saptanması
1978
AKÇORA, Ayhan | T.C. TARIM VE ORMAN BAKANLIĞI | YONGUÇ, Ali Demir | T.C. TARIM VE ORMAN BAKANLIĞI
English. This study was conducted to compare th e titration of sheeppox virus produced by Borrel method on sheep and sheep kidneytissue culture. 8 dilutions of the virus with 1/10.000 to1/1.280.000 were used for titrations.Results in sheep were appaered acrording to the pustulesand dose minimal reaction (DMR) after intadermicly injectionsof the vaccine virus dilutions. Any sheep refractory in theexper~ment were disc?trded. 21 .sheep were used in the study andthey were kıvırcık race which were more susceptible to thevirus.Results in the sheep kidney tissue culture were fallowed· for cytopathic efect (CPE) under microscope for 12 days. CPEwere appaered by the method of Reed and Muench and explainedby tissue culture infective dose (TCID/50).· Titrations on sheep were estımated as being with a rangeof 10-5,80 - 10-6,40 where as an tissue culture 10-5,65 10-6,35 the results of the both virus titrations were similar toeach other, but titrations of virus on sheep kidney tissueculture were obviosly more economic and practical than theregular method with pustules on sheep.
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