Morphology and anatomy of Gentiana lutea hairy roots
1998
Budimir, S. | Janosevic, D. | Momcilovic, I. | Grubisic, D. (Institute for Biological Research "Sinisa Stankovic", Belgrade (Yugoslavia))
Gentiana lutea L. hairy root clones, induced by Agrobacterium rhizogenes strain ATCC 15834, were thick, branched and with rare root hairs. All root clones tended to produce calluses in culture. This root phenotype differed from the typical one, which is characterized with thin, branched roots, with abundant root hairs. Histological examination demonstrated that cortical tissue of Gentiana lutea control roots consisted of spherical cells and small intercellular spaces, while in hairy roots enlarged, irregularly shaped cells and large intercellular spaces were usually observed. Along transformed roots, fasciated segments were frequently present. Multiple steles surrounded by cortical cells were observed on transverse sections of fasciated segments.
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