Amino acid analysis of a tumour - assotiated antigen isolated from the tumour tissue of cows with enzootic bovine leukosis
1998
Balent, P. | Nadova, P. | Kacmarik, J. (University of Veterinary Medicine, Kosice (Slovak Republic))
A tumour-associated antigen (TAA) expressed in tumour cells of cattle with enzootic bovine leukosis (EBL) was isolated from the tumour tissue of cows serologically and haematologically positive for EBL and patho-anatomically and histologically positive for lymphosarcomatosis of the prolymphocytic type. After homogenization, ultrasound disintegration and ultrahighspeed centrifugation of the tumour tissue, the glycoproteins of the supernatant were extracted using perchloric acid and the TAA was purified by gel filtration and successive SDS-polyacrylamide electrophoresis. The molecular weight of the TAA was estimated to be 70,000 D. By aminoacid analysis the total aminoacid content of TAA was found to be 5.935 mmol/g. The proportion of particular aminoacid groups was as follows: 74.8 neutral amino acids, 14.4 acidic amino acids, and 10.8 basic ones. Compared with non-tumour fractions the tumour fractions contained elevated amounts of glycine, alanine, isoleucine, leucine, and lysine.
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