Stem segment in vitro culture of Chrysanthemum as a method for micropropagation
2000
Radojevic, Lj. | Jevremovic, S. | Lazic, B. (Institut za bioloska istrazivanja "Sinisa Stankovic", Beograd (Yugoslavia))
The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in Yugoslavia was investigated. Medium supplemented with alfa-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar "Tigerrag". Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of in vitro plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.
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