Callous formation and regeneration of Sargasopsis zanardinii through culturing media
2000
Ebrahim zadeh, H. | Karimi, F. | Abhari, S.R.
Nowadays, seaweeds have many uses in food industry, weaving, pharmacy, medicine, biotechnology and so on. Many efforts has been made to regenerate seaweeds in artificial media in order to carry out genetic engineering process since 1970. In this study we have tried to establish a proper method for sterilization and tissue culture of brown seaweed, Sargasopsis zanardinii, in 1/2PES, PES and 2PES media. After sterilization, the explants of the seaweed were cultured in liquid and solid media. Consequently turgidity in margin of explants, longitudinal cell division and formation of hairy strands, and microcallous formation were showed by explants which cultured in PES and 2PES solid media. After transporting from liquid to solid media, juglike bodies were formed on the surface of solid PES media and then some brown strands were grown from these bodies that finally were flattened. None of these results was obtained from 1/2PES media.
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