Detection and evaluation of the genetic variation Litopenaeus vannamei for different seed

The aim of this study was to determin the genetic parameters, genetic differentiation and relative relationship between different generations of specific pathogenic free of L.vannamei via molecular methods. Tissue samples of shrimp were randomly get caught from the different area. Genetic parameters were evaluated by 12 special primers of L.vannamei via microsatellite assay. In this study, two population of Molokaei and High health were identified as shrimp broodstocks of specific disease free (SPF) zero generation. Breeding among shrimp (males and females) were through inbreeding and crossbreeding. Three various stocks were produced: H♂ × M♀, M♂ × H♀ and H♀ × H♂. Genetic differences and relative relative relationship between different generations of specific pathogenic free shrimp were to evaluate by evaluation of 16S rRNA and d-loop region mitochondrial of different generations, respectively. The results showed that significant differences were observed among genetic distance of different populations of L.vannamei farmed (0.615). There were o.o78 Fst value of M.H and H.M stokes that it was a significant difference (P <0.001). The analysis of d-loop region of mitochondrial genomes of different generations shrimp was observed 997 positions with 3-6 and 766-799 of haplotype and monomorphic, respectively. Despite haplotype diversity and nucleotide diversity of all generations SPF shrimp were 0.877 and 0.12, respectively. While, Base on result of 16S rRNA indicated that of 486 sites have been identified 484 sites were conserved. Also, monomorphic sites ranged between 482-486 sites and was consists 2 polymorphic sites and 2 transitional sites. The number haplotypes, haplotypes diversity, nucleotide diversity revealed in this region were 2, 0.356±0.159 and 0.00147, respectively. Regarding the results of in this study can be concluded that lack of arriving new broodstocks from outside the country and was mating between adult males and females relative (full-sib) addition to small effective population size there was both genetic drift and number of haplotypes was gradually reduced from zero generation to second generation. Hence, due to the high close relationship between different generations shrimp evaluation of d-loop and 16S rRNA regions of the mitochondrial genome were little genetic difference between them.

Iranian Fisheries Science Research Institute

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