Evolution close relationship between broodstock and seeds of Litopenaeus vannamei each generation
2017
Pazir, M.Kh. | Ghasemi, A. | Ghaednia, B. | Aeinjamshid, Kh. | Pourkazemi, M. | Matinfar, A. | Afsharnasab, M. | Qharibi, Gh. | Safavi, E. | Zendehbodi, A.A. | Modaresi, M. | Khamyab, M. | Faghih, A.
In this study, two population of Molokaei and High health were selected as zero generation of Litopenaeus vannamei broodstocks. After mating males and females adult within inbreeding and crossbreeding three various storage were produced: H♂ × M♀, M♂ × H♀ and H♀ × H♂. The second generation of shrimp were obtained from storage of broodstocks (MH) first generation. The aim of this study was investigated close relationship between different generations of specific pathogenic free shrimp. The analysis d-loop region of mitochondrial genomes of different generations shrimp was observed 997 positions with 3-6 and 766-799 of haplotype and monomorphic, respectively. Despite haplotype diversity and nucleotide diversity of all generations SPF shrimp were 0.877 and 0.12, respectively. But, results showed homozygosity rate is increasing from one generation to next generation, so low genetic distance were observed between different generations of SPF shrimp certain disease-free shrimp. Regarding the results of in this study can be concluded that lack of arriving new broodstocks from outside the country and was mating between adult males and females relative (full-sib) addition to small effective population size there was both genetic drift and number of haplotypes was gradually reduced from zero generation to second generation. Hence, due to the high close relationship between different generations shrimp evaluation of d-loop region of the mitochondrial genome were little genetic difference between them
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