APPLICATION OF DOT-IMMUNOASSAY FOR INDICATION OF RABIES ANTIGEN IN PATHOLOGICAL MATERIAL | ПРИМЕНЕНИЕ ДОТ-ИММУНОАНАЛИЗА ДЛЯ ИНДИКАЦИИ РАБИЧЕСКОГО АНТИГЕНА В ПАТОЛОГИЧЕСКОМ МАТЕРИАЛЕ
2023
R. M. Akhmadeev | A. F. Arslanova | N. R. Miftahov | S. M. Nasirov | M. A. Efimova | I. I. Samerkhanov | Р. М. Ахмадеев | А. Ф. Арсланова | Н. Р. Мифтахов | Ш. М. Насыров | М. А. Ефимова | И. И. Самерханов
The aim of the research was to develop a protocol for an indirect dot-immunoassay on a nitrocellulose membrane (NCM) for the qualitative determination of the presence of rabies virus antigen in pathological material. As the test samples, we used brain samples of various animal species (foxes and mice), which were positive during the initial study by the methods of fuorescent antibodies and bioassays. Te setting of the immunofuorescence reaction was carried out according to GOST 26075-13 with some modifcations. Immunofuorescence reaction and indirect «sandwich» ELISA (enzyme-linked immunosorbent assay) were carried out using diagnostic kits manufactured at the FSBSI «FCTRBS- ARRVI»(Kazan). Te materials present the results of primary laboratory tests of the test system for the indication of rabies antigen based on the indirect dot-immunoassay of 81 samples of pathological material from the brain of various animal species. It was shown that indirect dot-immunoassay has 100 % specifcity and a sensitivity of at least 96.3 %, and its optical signals correlate with the results of indirect ELISA. Te proposed indirect dot-immunoassay method, in addition to independent use, can also serve as a basis for the design of test systems based on immunochemical analysis.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Don State Technical University