The fermented non-digestible fraction of spent coffee grounds induces apoptosis in human colon cancer cells (SW480)

Maldonado Celis, María Elena; García Gutiérrez, Nataly; Rojas López, Mauricio; Loarca Piña, Guadalupe Flavia; Campos Vega, Rocío; Impacto de Componentes Alimentarios en la Salud

The fermented non-digestible fraction of spent coffee grounds induces apoptosis in human colon cancer cells (SW480)

2017

ABSTRACT: Gut flora-mediated non-digestible/unabsorbed fraction of spent coffee grounds (hgf-NDSCG) was evaluated for its chemopreventive effect and molecular mechanisms involved on human colon adenocar- cinoma SW480 cell survival. Small intestinal digestion released bioavailable ascorbic, chlorogenic and gallic acids from hgf-NDSCG reducing oxidative stress. The hgf-NDSCG inhibited SW480 growth (LC50-19%) dose dependently by decreasing glutathione/oxidized glutathione (GSH/GSSG) ratio, indicat- ing a cellular oxidative stress process. Flow cytometry analysis demonstrated the presence of apoptotic cells in a hypodiploid sub G0/G1-peak as a consequence of partial DNA loss. The pro-apoptotic mechanism of hgf-NDSCG was confirmed by SW480 cell toxicity induced by increased Caspase-3 activity and mitochondria dysfunction modulating oxidative stress using Annexin V and DiOC2(3) staining assays. The chemopreventive effects of spent coffee grounds (SCG) indicate that polyphenols present in hgf-NDSCG, digested and fermented by colon microbiota, can inhibit survival of human colon cancer cell and offer effective prophylactic value to prevent colon cancer.

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COL0083811

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AGROVOC Keywords

adenocarcinoma antioxidantes antioxidants apoptosis

Bibliographic information

Publisher

International Society for Nutraceuticals and Functional Foods (ISNFF); Elsevier, Ámsterdam, Países Bajos

Other Subjects

Puntos de control del ciclo celular; Neoplasias del colon; Cell cycle checkpoints; Colonic neoplasms

Language

English

Format

10, application/pdf, application/pdf

License

https://creativecommons.org/licenses/by-nc-nd/4.0/, http://creativecommons.org/licenses/by-nc-nd/2.5/co/, info:eu-repo/semantics/openAccess, http://purl.org/coar/access_right/c_abf2

ISSN

1756-4646, 2214-9414

Type

Artículo De Investigación; Http://purl.org/coar/resource_type/c_2df8fbb1; Https://purl.org/redcol/resource_type/art; Http://purl.org/coar/version/c_970fb48d4fbd8a85; Info:eu-Repo/semantics/article; Info:eu-Repo/semantics/publishedversion

In AGRIS since: 2024-10-18

Modification date: 2025-05-22

Format: Dublin Core

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Links

DOI DOI https://hdl.handle.net/10495/36185

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