Vkorc1 gene genotypes for Rattus norvegicus and Rattus rattus in the City of Barcelona
2024
Ruiz-López, María José | Franco, Sandra | Martínez de la Puente, Josué | Ferraguti, Martina | Miccolis, Emanuele | Petit, Robert | Barahona, Laura | Figuerola, Jordi | Montalvo, Tomás | Agència de Salut Pública de Barcelona ASPB | Ruiz-López, María José [0000-0002-6849-644X] | Martínez de la Puente, Josué [0000-0001-8055-4115] | Figuerola, Jordi [0000-0002-4664-9011]
[Description of methods used for collection/generation of data] Brown rats were sampled from the sewage system during two periods: from December 2016 to November 2017 when difenacoum and brodifacoum were used, and from August 2021 to July 2022 when bromadiolone was used. Because black rats have just recently been detected in Barcelona, we only studied them during the latter sampling period, with samples obtained from a control action carried out in a green urban area. Exon 3 of the Vkorc1 gene was characterized in both species, while exon 1 was additionally analysed in black rats. Synonymous mutations, not resulting in amino-acid changes, were found in both exons, indicating no evidence of anticoagulant resistance in the rats of Barcelona. [Methods for processing the data] Sequences were analyzed using Geneious v. 2020.0.3 (Kearse et al., 2012).
Show more [+] Less [-]Agència de Salut Pública de Barcelona.
Show more [+] Less [-]VKORC1_Rattussp_mutations.xlsx
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