Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
2004
Mateo, Rafael | Castells, Gloria | Green, Andy J. | Godoy, Carlo | Cristòfal, Carles
Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards
Show more [+] Less [-]Peer reviewed
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Estación Biológica de Doñana
