Gene and protein expression for prolactin, growth hormone and somatolactin in Sparus aurata: Seasonal variations
2009
Vargas-Chacoff, Luis | Astola, A. | Arjona, F. J. | Martín del Río, María del Pilar | García-Cozár, F. | Mancera, Juan Miguel | Martínez-Rodríguez, Gonzalo
6 páginas, 4 figuras, 2 tablas.
Show more [+] Less [-]The seasonal variation of PRL, GH and SL gene and protein expression has been analyzed in gilthead sea bream (Sparus aurata) pituitaries using Real-Time Q-PCR and Western Blots, respectively. Animals were cultured in earthen ponds under natural photoperiod, temperature and salinity conditions. Samples were taken during winter 2005 (January), spring 2005 (April), summer 2005 (July) and autumn 2005 (October). β-actin, used as the housekeeping gene both for Q-RT-PCR and Western analysis, did not present significant differences among seasons. Higher expression was observed during spring and autumn for PRL, summer and winter for GH, and spring for SL. Expression of PRI, GH and SL, presented seasonal variation, suggesting that these hormones could play a role in the molecular signal transduction of environmental factors (especially of photoperiod and temperature) in eurythermal fish.
Show more [+] Less [-]This study was partly supported by grants AGL2007-61211/ACU (Ministerio de Educación y Ciencia and FEDER, Spain) and Proyecto de Excelencia PO7-RNM-02843 (Junta de Andalucía) to J.M.M. The authors wish to thank Cupimar S.L. (El Puerto de Santa María, Cádiz) for providing the experimental fish. L.V.C. is funded by the program Beca Presidente de la República de Chile and Comisión de Ciencia y Tecnología de Chile (CONICYT).
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