Optimizing delignification and pitch removal by treating eucalypt pulp with Myceliophthora laccase and a phenolic mediator
2012
Rico Campos, Alejandro | Babot, Esteban Daniel | Rencoret, Jorge | Río Andrade, José Carlos del | Martínez, Ángel T. | Gutiérrez Suárez, Ana
This work shows the ability of two natural phenols, namely syringaldehyde and methyl syringate, to act as mediators of recombinant Myceliophthora thermophila laccase (MtL) in eucalypt-pulp delignification. After alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved with respect to the control. Likewise, removal of the main lipophilic extractives present in eucalypt pulp, such as free and conjugated sterols, was observed after the above laccase-mediator treatments. In the same study, the doses of both MtL and methyl syringate were reduced, and results compatible with industrial implementation were obtained, taking also advantage from the high-yield production of the recombinant enzyme that enables its commercial availability.
Show more [+] Less [-]This study was funded by the BIORENEW and LIGNODECO EU-projects (NMP2-CT-2006-026456 and KBBE-2009-3-244362, respectively). Novozymes and ENCE are acknowledged for laccase and eucalytp pulp supply, respectively. E.D. Babot and J. Rencoret thank the CSIC for a JAE fellowship and JAE-Doc contract, respectively, of the program “Junta para la Ampliación de Estudios” co-financed by the European Social Fund (ESF).
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