Improved methods for recovering eggs of Toxocara canis from soil
2000
Ruiz de Ybáñez Carnero, María del Rocío | Garijo Toledo, María Magdalena | Goyena Salgado, Marina | Alonso de Vega, Francisco Domingo | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
©2000 Cambridge University Press. This document is the Published version of a Published Work that appeared in final form in Journal of Helminthology. To access the final edited and published work see https://doi.org/10.1017/S0022149X00000512
Show more [+] Less [-]The ingestion of soil in parks and public places containing eggs of Toxocara may constitute a significant health risk, particularly to children. To determine the most efficient method for extracting eggs from experimentally contaminated soil, two consecutive studies were undertaken. Four techniques, including washing, sieving, vacuum, and the one recommended by the World Health Organization, were evaluated. Recovery rates of over 85% were recorded with both washing and sieving methods. Using the washing technique, all combinations of the four pre-treatment solutions, distilled water, acetoacetic solution pH 5, 0.1 N sodium hydroxide and 1% Tween 20, and seven flotation fluids with different speci®c gravities (S.G.) ranging from 1.20 to 1.35 were assayed. The association of distilled water and saccharose solution with an S.G. of 1.27 showed the best results, with a recovery rate of 99.91%.
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