Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas
2018
Couso Liáñez, Inmaculada Concepción | Pérez Pérez, María Esther | Martínez Force, Enrique | Kim, Hee-Sik | Crespo González, José Luis | Ministerio de Economía y Competitividad (MINECO). España | Junta de Andalucía
Autophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material to maintain cellular homeostasis. This highly dynamic process is characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver the cargo to the vacuole. Flow of material through the autophagy pathway and its degradation in the vacuole is known as autophagic flux, and reflects the autophagic degradation activity. A number of assays have been developed to determine autophagic flux in yeasts, mammals, and plants, but it has not been examined yet in algae. Here we analyzed autophagic flux in the model green alga Chlamydomonas reinhardtii. By monitoring specific autophagy markers such as ATG8 lipidation and using immunofluorescence and electron microscopy techniques, we show that concanamycin A, a vacuolar ATPase inhibitor, blocks autophagic flux in Chlamydomonas. Our results revealed that vacuolar lytic function is needed for the synthesis of triacylglycerols and the formation of lipid bodies in nitrogen- or phosphate-starved cells. Moreover, we found that concanamycin A treatment prevented the degradation of ribosomal proteins RPS6 and RPL37 under nitrogen or phosphate deprivation. These results indicate that autophagy might play an important role in the regulation of lipid metabolism and the recycling of ribosomal proteins under nutrient limitation in Chlamydomonas
Show more [+] Less [-]España, MINECO BFU2015-68216-P
Show more [+] Less [-]España, Junta de Andalucía CVI-7336 (to JLC), BIO2015-74432-JIN (to MEPP)
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Universidad de Sevilla
