Cytotoxic Effects and Oxidative Stress Produced by a Cyanobacterial Cylindrospermopsin Producer Extract versus a Cylindrospermopsin Non-Producing Extract on the Neuroblastoma SH-SY5Y Cell Line
2023
Hinojosa Hidalgo, María Gracia | Cascajosa Lira, Antonio | Prieto Ortega, Ana Isabel | Gutiérrez-Praena, Daniel | Vasconcelos, Vitor | Jos Gallego, Ángeles Mencía | Cameán Fernández, Ana María | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | Universidad de Sevilla. CTS358: Toxicología | Universidad de Sevilla. AGR258: Alimentos Funcionales e Investigación Toxicológica | Spanish Ministerio de Economía y Competitividad and FEDER AGL2015-64558-R | Spanish Ministerio de Ciencia e Innovación PID2019-104890RBI00/AEI/10.13039/501100011033 | Ministerio de Universidades (Spain) FPU2019/01247 | Universidad de Sevilla (Spain) VII Plan Propio de Investigación y Transferencia—US 2022/00000298
The incidence and interest of cyanobacteria are increasing nowadays because they are able to produce some toxic secondary metabolites known as cyanotoxins. Among them, the presence of cylindrospermopsin (CYN) is especially relevant, as it seems to cause damage at different levels in the organisms: the nervous system being the one most recently reported. Usually, the effects of the cyanotoxins are studied, but not those exerted by cyanobacterial biomass. The aim of the present study was to assess the cytotoxicity and oxidative stress generation of one cyanobacterial extract of R. raciborskii non-containing CYN (CYN−), and compare its effects with those exerted by a cyanobacterial extract of C. ovalisporum containing CYN (CYN+) in the human neuroblastoma SH-SY5Y cell line. Moreover, the analytical characterization of potential cyanotoxins and their metabolites that are present in both extracts of these cultures was also carried out using Ultrahigh Performance Liquid Chromatography-Mass Spectrometry, in tandem (UHPLC-MS/MS). The results show a reduction of cell viability concentration- and time-dependently after 24 and 48 h of exposure with CYN+ being five times more toxic than CYN−. Furthermore, the reactive oxygen species (ROS) increased with time (0–24 h) and CYN concentration (0–1.11 µg/mL). However, this rise was only obtained after the highest concentrations and times of exposure to CYN−, while this extract also caused a decrease in reduced glutathione (GSH) levels, which might be an indication of the compensation of the oxidative stress response. This study is the first one performed in vitro comparing the effect
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