Methods for extraction, processing and detection of plant and soil nematodes
2005
Hooper, David J. | Hallmann, Johannes | Subbotin, Sergei A.
Diagnosis of nematode damage requires methods for their extraction, handling and detection. The methods take advantage of size, density and motility of the nematodes to separate them from plant tissue and soil particles by means of sieving, centrifugation and filtration. Different methods allow different applications such as for diagnosis, determination of infestation levels, monitoring nematode populations and statutory testing for the presence of quarantine nematodes. Besides morphology and morphometrics, molecular techniques are increasingly used for rapid and accurate identification of nematodes. This chapter gives details of the most common methods. There are many modifications to the basic methods often determined by local supplies of equipment and operating conditions. Further references with excellent sections on methodology are: Ayoub (1980), Dropkin (1989), Hunt and De Ley (1996), Shurtleff and Averre (2000), Southey (1986), Thorne (1961) and Zuckerman et al. (1985).
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Publisher CABI Publishing
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