Honey bee Biodiversity in Western Black Sea and Evidence for a new Honey bee Ecotype in Yığılca Provinces of Düzce city
2019
KEKECOĞLU, Meral
The genetic variability of honey bee (Apis mellifera L.) populations of Western Black Sea were comparatively investigated by using geometricmorphometric analysis and mtDNA marker. For this study, 650 young worker honey bees were collected from 13 different areas of Western BlackSea. The geometric morphometrics analysis was done using the cordinates of 18 landmarks located at vein intersections of the fore right wing.Genetic structure of these honey bee populations were also studied by means of RFLP analysis of two mtDNA gene segment. Total DNAwas extracted, then 16s rDNA (965 bp) and COI (1028 bp) were amplified using PCR and sequencely tested for SspI and DraI restriction enzymeprofiles.Both SspI restriction polymorphism of COI gene segment of mtDNA and Principal Component analysis of the cordinates of 18 landmarksclearly showed that endemic honey bees of Yigilca province in Düzce discriminated from the other population of Western Black Sea. As the results,endemic honey bees of this province were described as a different ecotype.
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