COMPARISON OF THE ANTI-CARCINOGENIC EFFECTS OF SOME PROBIOTIC BACTERIA AND THEIR POSTBIOTICS ON COLORECTAL CANCER CELLS
2022
Nigdelioglu Dolanbay, Serap | Aslim, Belma
The third most widespread cancer and the second leading reason for cancer-associated death is colorectal cancer (CRC). Natural agents such as probiotics and postbiotics, that offer anti-carcinogenic effects for CRC prevention, have become an important focus in recent years. Therefore, the aim of this study is to compare the anti-proliferative effects related to anti-genotoxic and immunomodulatory effects of viable probiotics with their exopolysaccharides (EPSs), which is one of their postbiotics. For this purpose, the strains' ability to inhibit the proliferation of HT-29 cells were determined with the WST-1 assay kit, their genotoxic and anti-genotoxic effects with the Comet assay and their immunomodulatory effects with IL-8 and IL-10 ELISA kits. According to our results, both viable probiotics and lyophilized EPSs (L-EPSs) were effective in all studies, but the best anti-proliferative (51% cell death), anti-genotoxic (48% inhibition) and immunomodulatory (for IL-8: 46% suppression and for IL-10: 74% increase) (*p < 0.05) effect was obtained from viable probiotics (Levilactobacillus brevis LB63). Additionally, in the present study found that these effects of L-EPSs were close to viable probiotics. Therefore, it has been shown that postbiotics can be used as alternatively to viable probiotics, because of the properties such as reliable and no side effects of their, thus it may be a useful alternative for cancer. According to these results, new agents such as probiotic-based postbiotics will be introduced to the pharmaceutical and food industry as well as probiotic bacteria that are protective and/or therapeutic against cancer.
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