Met-Enkephalin-Like Peptides and ghrelin mitigate negative effects of bariatric surgery in rats

English. Endogenous opioid peptides and ghrelin are synthesized in the gastro-intestinal tract and modulate its functions in human and animals in physiological and pathophysiological conditions. Opioids, mainly Met-enkeph- alin, and ghrelin are involved into regulation of appetite, gastric emptying, intestinal motility and intestinal juices secretion. Obesity is a metabolic disorder of energy homeostasis caused by the overdose of energy contained in food in relation to the needs of the organism. Bariatric surgery is often considered as a method of obesity treatment being more effective with a longterm. However, it seems probable that surgery markedly affects the interaction between these two endogenous peptides and their receptors. Thus the aim of the study was to compare the effect of two methods of bariatric surgery on the concentrations of Met-enkephalin and ghrelin in the stomach and other regions of the gastro-intestinal tract in rats. Experimentation was undertaken using 24 male Wistar rats divided into control and two experimental groups undergoing sleeve gastrectomy (GR) or gastric plication (GP). Three weeks after surgery blood and fragments of stomach, duode- num, jejunum, ileum and ascending colon were collected. Plasma glucose and triglycerides were significantly changed after the bariatric surgery. Both bariatric procedures significantly but differentially changed the Met-enkephalin and ghrelin concentrations in the most of tested tissues. The obtained results clearly showed involvement of Met-enkephalin and ghrelin in the regulation of post-bariatric metabolic processes.

English. Met-enkephalin, ghrelin, bariatric rats, gastro-intestinal

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