Bacterial contamination in the waters of two lake and river systems in NE Poland in relation to the physicochemical properties of water

Zdigitalizowano i udostępniono w ramach projektu pn. Rozbudowa otwartych zasobów naukowych Repozytorium Uniwersytetu w Białymstoku – kontynuacja, dofinansowanego z programu „Społeczna odpowiedzialność nauki” Ministra Edukacji i Nauki na podstawie umowy BIBL/SP/0040/2023/01.

Determination of the sanitary-epidemiological status of waters used for tourism purposes is very important, hence the aim of this research was to determine the sanitary status of various types of surface water used for recreation. The research area included 18 stand s on the Ostróda-Elbląg Canal and 25 on the Augustów Canal. Water samples were taken during the summer period in 2017-2018. Microbiological analyses were performed in accordance with PN-EN ISO standards and included determining the total abundance of such bacteria as E. coli, coliforms, fecal enterococci and P. aeruginosa. In addition, basic physicochemical analyses of water were performed according to standard methods. The presence of indicator bacteria in the water indicates their fecal contamination and possible epidemiological threat. The general average abundance of bacteria for the waters of the Augustów Canal was 1,628,000±784,000 CFU /mL, while for the Ostróda-Elbląg Canal 2,242,800±964,300 CFU /mL. The abundance of E. coli bacteria in the waters ranged from O CFU / 100mL to 6400 CFU/100mL, coliforms from O CFU/100mL to 7,200 CFU/ 100mL, fecal enterococci from O to 5,800 CFU /100mL. The maximum values of these indicators were recorded in lakes. The P. aeruginosa bacteria were isolated only from waters from an artificial section of the river-lake systems, and their abundance fluctuated in the 120-860 CFU /100mL range. Statistical analyses have shown that the abundance of indicator bacteria depends on some physicochemical parameters of water, such as: EC, pH, temperature, total carbon concentration, total nitrogen concentration. Summing up, it can be concluded that the highest values of the abundance of indicator bacteria occurred in lakes. The river sections were characterized by higher values of an abundance of coliforms and E. coli bacteria and the total abundance of bacteria compared to canal waters, which indicates a fresh inflow of pollutants to the tested systems. In tum, the canal sections were the only habitat of P. aeruginosa.

A. Cudowski: cudad@uwb.edu.pl

Anna Pietryczuk - Department of Hydrobiology, Institute of Biology, University of Bialystok

Adam Cudowski - Department of Hydrobiology, Institute of Biology, University of Bialystok

Adewoye, S. O. (2010) Effects of detergent effluent discharges on the aspect of water quality of ASA River, Ilorin, Nigeria. Agriculture and Biology Journal of North America, 1(4):731-36.

Anderson, K.L., Whitlock, J.E., Harwood, V.J. (2005) Persistence and Differential Survival of Fecal Indicator Bacteria in Subtropical Waters and Sediments. Appl. Environ. Microb., 71(6): 3041-48.

Augustynowicz, J., Nierebiński, M., Zawada, M., Zielonka D., Russel, S. (2015) The impact of basic physicochemical parameters on microbiological water quality on a selected section of the Vistula. Woda - Środowisko - Obszary Wiejskie, 15(1): 5-15 (in Polish).

Barakat, A, Baghdadi, M.E., Meddah R., Rais, J., Nadem S., Afdali, M. (2013) Evaluation of water quality in open channels flowing through Beni-Mellal City (Morocco). Journal of Water and Land Development, 19: 3-11.

Biedka, P., Wawrentowicz, D. (2011) The role of tributaries in shaping the quality of the lakes of the Suwałki-Augustów Lake District. Inżynieria Ekologiczna, 26: 7-17 (in Polish).

Boehm, A.B., Sassoubre, L.M. Enterococci as Indicators of environmental fecal contamination. [In:] Gilmore, M.S., Clewell, D.B., Ike, Y., et al., (eds.) Enterococci: From commensals to leading causes of drug resistant infection [Internet]. Boston: Massachusetts Eye and Ear Infirmary, 2014: pp. 1-21.

Briée, C., Moreira, D., López-Garcia, P. (2007) Archaeal and bacterial community composition of sediment and plankton from a suboxic freshwater pond. Res. Microb., 158(3): 213-27.

Bradshaw, J.K, Snyder, B.J., Oladeinde, A. Spidle, D., Berrang, M. E., Meinersmann, R. J, Oakley, B., Sidle, R C., Sullivan, K., Molina, M. (2016) Characterizing relationships among fecal indicator bacteria, microbial source tracking markers, and associated waterborne pathogen occurrence in stream water and sediments in a mixed land use watershed. Water Res., 101: 498-509.

Byappanahalli, M.N., Nevers, M.B., Korajkic A., Staley, Z.R., Harwood V.J. (2012) Enterococci in the Environment. Microbiol. Mol. Biol. Rev., 76(4): 685-706.

Cabral, J. P. S. (2010) Water microbiology. Bacterial pathogens and water. Int. J. Environ. Res. Public Health, 7: 3657-703.

Chomutowska, H. (2009) The influence of selected physicochemical factors on the Rospuda river's bacterioplankton. Ochrona środowiska i Zasobów Naturalnych, 40: 513-523 (in Polish).

Fogarty, L.R, Haack, S.K., Wolcott, M.J., Whitman, R.L. (2003) Abundance and characteristics of the recreational water quality indicator bacteria Escherichia coli and enterococci in gull faeces. J. Appl. Microb., 93(5): 865-878.

Furgała-Selezniow, G., Turkowski, K., Nowak, A., Skrzypczak, A., Mamcarz, A. (2006) The Ostroda-Elblag Canal in Poland: The Past and Future for Water Tourism. [In:] Hall, C.M., Härkönen T. (eds) Lake Tourism. An integrated approach to lacustrine tourism system, Chris Cooper (University of Queensland, Australia), 2006: pp. 131-148.

Hijosa-Valsero, M., Bécares, E., Fernandez-Aláez, C., Fernandez-Aláez, M., Mayo, R., Jiménez, J.J. (2016) Chemical pollution in inland shallow lakes in the Mediterranean region (NW Spain): PAHs, insecticides and herbicides in water and sediments. Scienc. Total Environ., 544: 797-810.

Hillbricht-Ilkowska, A., Wiśniewski, R. J. (1996) The functioning of river - lake systems in the post-Iake landscape: the Krutynia River (Mazury Lake District). Zeszyty Naukowe Komitetu "Człowiek i Środowisko", 13: 1-461. (in Polish).

Hoyer, M.V., Donze, J.L., Schulz, E.J, Willis, D.J., Canfield, D.E. (2006) Total coliform and Escherichia coli Counts in 99 Florida Lakes with Relations to Some Common Limnological Factors. Lake and Reservoir Management, 22(2):141-50.

Ihejirika, C.E., Ogbulie, J.N., Nwabueze, R.N., Orji, J.C., Ihejirika, O.C., Adieze, I.E., Azubike, O.c., Ibe I.J. (2011) Seasonal influences on the distribution of bacterial pathogens and waterborne diseases transmission potentials of Imo river, Nigeria. J. Res. Biol., 1 (3): 163-72.

Kundzewicz, Z. W., Zalewski, M., Kędziora, A., PierzgaIski, E. (2010) Risks associated with water. Nauka, 4: 87-96 (in Polish).

Layton, B.A., Walters, S.P., Lam, L.H., Boehm, AB. (2010) Enterococcus species distribution among human and animaI hosts using multiplex PCR J. Appl. Microbiol., 109(2): 539-74.

Marcheggiani, S., D'Ugo, E., Puccinelli, C., Giuseppetti, R., D'Angelo, A.M., Gualerzi, C.O., Spurio, R., Medlin, L.K., Guillebault, D., Weigel, W., Helmi, K., Mancini, L. (2015) Detection of emerging and re-emerging pathogens in surface waters close to an urban area. Int. J. Environ. Res. and Public Health, 12 (5):5505-27.

McLellan, S.L., Hollis E.J., Depas M.M., Van Dyke, M., Harris, J., Scopel, C.O. (2007) Distribution and fate of Escherichia coli in Lake Michigan following contamination with urban stormwater and combined sewer overflows. Journal of Great Lakes Research, 33(3): 566-80.

Mena, K.D., Gerba, Ch.P. (2009) Risk assessment of Pseudomonas aeruginosa in water. Rev. Environ. Contamin. Toxicol., 201:71-115.

Newton, R.J., Stuart, E.J, Eiler, A., McMahon, K. D., Bertisson, S. (2011) A guide to the naturaI history of freshwater lake bacteria. Microbiol. Mol. Biol. Rev., 75:14-49.

Niewolak, S., Opieka, A. (2000) Potentially pathogenic microorganisms in water and bottom sediments in the Czarna Hańcza River. Pol. J.Environ.5tud., 9 (3):183-94.

Sampson, R.W., Swiatnicki, S.A., Osinga, V.L., Supita, J.L., McDermott, C.M., Kleinheinz, G.T. (2006) Effects of temperature and sand on E. coil survival in a northern lake water microcosm. Journal of Water and Health, 4(3): 389-93.

Siuda, W., Chrust, R. J. (2002) Oecomposition and utilization of particulate organic matter by bacteria in lakes of different trophic status. Pol. J. Environ. Stud, 11: 53-65.

Starliper, C.E., Watten, B.J., Iwanowicz, D.D., Green, P.A., Bassett, N.L, Adams, C.R. (2015) Efficacy of pH elevation as a bactericidal strategy for treating ballast water of freight carriers. J. Advanc. Res., 6: 501-509.

Suchowolec, T., Górniak, A. (2006) Seasonality of water quality in small retention reservoirs in the Podlasie agricultural landscape. Woda - Środowisko - Obszary Wiejskie, 6(2): 347-58 (in Polish).

Vignesh, S., Muthukumar, K., Santhosh Gokul, M., James, R.A. (2013) Microbial pollution indicators in the Cauvery River, Southern India. [In:] On a sustainable future of the earth's natural resources. Springer Earth System Sciences, 20:364-76.

Wetzel, R. G. Bacterioplankton. Limnology: Lake and River Ecosystems, 3rd ed. New York: Academic Press, 2001: pp. 489-525.

Zehr, J.Z., Ward, B.B. (2002) Nitrogen cycling in the ocean: New perspectives on processes and paradigms. Appl. Environ. Microb., 68(3): 1015-24.

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