Pathological study of alcoholic plant agolanceolata crude extracts effect on some Salmonella species invitro and in vivo

This study was designed to explore the pathological effect of alcoholic plant agolanceolata crude extracts effect on some Salmonella species invitro and in vivo study by using laboratory mice .plant agolanceolata  crude extract were dealing in vitro study against some virulent bacteria including Salmonella typhimurium ,Salmonella typhiond Salmonella hadar. And using the concentrations (100,150,200,250 and 300) mg for plan tagolanceolata crude extract ,concentration at 300 mg and the highest inhibitory effect on the three types of salmonella ,when compared with the other concentrations .The pathological study of plant agolanceolata crude extract was done against the infection of Salmonella typhimurium as in vivo study by using white laboratory mice. Twenty four mice were randomly divided into six groups, each group contain four animals. First group was administrated orally o.3 ml of Salmonella typhimuriumof bacterial suspension containing 1x106cfu orally for one week of infection. Second group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally of Salmonella typhimurium for two weeks as infection. Third group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally Salmonella typhimurium for three weeks as infection. Fourth group was administrated orally with o.3 ml of plant agolanceolata  extract for one week daily after infection with Salmonella typhimurium. Fifth group was administrated orally with o.3 ml of plant agolanceolata  extract for two weeks daily after infection with Salmonella typhimurium. And sixth group was administrated orally with o.3 ml of plant agolanceolata  extract for three weeks daily after infection with Salmonella typhimurium. The histopathological study showed pathological changes in theintestine of the first   , second and third groups that infected with Salmonella typhimuriumbacteria, (sixfhgroup)no clear pathological changes were reported except some lesions.  For all, the plantagolanceolata extract apparently has therapeutic effect whenused in high concentration invitro and for long time invivo.