Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver and their ability to expoxidize aldrin
1985
Rosli bin Mohamad (Universiti Pertanian Malaysia, Serdang, Selangor. Dept. of Agronomy and Horticulture)
Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were assayed for cytochrome P-450 content and aldrin epoxidae activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7-9 times compared to that of the rat liver. Epoxidae activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide
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