Quantitative determination of 4-methylthio-3-butenylisothiocyanate in radish root by RP-HPLC
1986
Kim, M.R. (Chungnam National Univ., Taejon (Korea R.)) | Rhee, H.S. (Seoul National Univ., Seoul (Korea R.). Dept. of Food and Nutrition)
RP-HPLC (Reverse phase-high performance liquid chromatography) assay which was introduced to measure quantitatively the amount of 4-methylthio-3-butenyl isothiocyanate in the radish root, proved to be convenient, accurate and reproducible, showing a good linearity between 10n moles/ml and 120 n moles/ml (r=0.9997). The results of this assay showed that while the isohtiocyanate was hydrolyzed slowly in the basic medium, it decomposed rapidly in the acidic aqueous medium
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