A survey of viral diseases of imported orchids in the Philippines and new local lesion hosts of tobacco mosaic virus orchid strain (TMV-O) and cymbidium mosaic virus (CYMV)
1985
Palacpac, M.B.
The survey of viral diseases of newly-arrived imported orchids at the port of entry revealed that 10.84 percent of the total number of orchid plants that came in from July, 1983 to November, 1984 were infected with either the orchid strain of tobacco mosaic virus (TMV-O) or Cymbidium mosaic virus (CyMV) singly or in combination with each other. The orchid strain of tobacco mosaic virus (TMV-O) was identified through the characteristics reddish brown local lesions produced on Gomphrena globosa 3 to 5 days after inoculation. Electron microscopy showed TMV-O as a rigid rod like particle which measured 285-325 x 15-20 nm. Mechanical transmission of Cymbidium mosaic virus (CyMV) on Datura stramonium produced necrotic brown lesions, 4 to 5 mm in diameter, 12 to 15 days after inoculation. Local lesions first appeared on the oldest inoculated leaves (12 to 14 days after inoculation) and last on the youngest leaves (22 days). Electron micrographs showed it to be long, slender, flexuous rods with measurements of 475 nm x 12-14 nm. A number of orchid plants were found to be doubly infected with TMV-O and CyMV. Solanum melongena L., was found to be a new indicator host of TMV-O. Grayish to black lesions, about 4 to 5 mm in diameter, were produced on the inoculated leaves 3 to 5 days after inoculation. Eight varieties of eggplant studied produced symptoms when inoculated with TMV-O
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