Selection of mutant variants in rice resistant to Xanthomonas oryzae by tissue culture 2. Variations of characters in progenies of regenerated plants
1989
Sun Lihua | Lu Xuefeng | Tang Banggen (Jiangsu Provincial Academy of Agricultural Sciences, Nanjing (China). Inst. of Agrobiological Genetics and Physiology)
Calli from dehulled seeds of Nangeng 34 (Oryza sativa subsp. japonica), a rice variety susceptible to bacterial leaf blight (Xanthomonas oryzae) were co-cultured with Xanthomonas oryzae pathogen in order to establish cell clones and regenerate plants resistant to the disease. The same kind of calli were also cultured in the absence of the pathogen as a control. 122 and 53 regenerated plants were obtained from the co-cultured calli and the control respectively, which had 94.2% and 51.9% of their respective totals showing various phenotypical variations, including disease resistance, polyploidy, sterility, more seeds per panicle, lower or higher seed weight, shorter plants and panicles, shorter and narrower flag leaves, longer growth duration, compacter plant type, straight and erect leaves. This indicates that variation frequencies in the regenerated plants were significantly increased by co-culture of the calli with the pathogen and its secretion. The performances of the selfed progenies (R1 - R5) of the regenerated plants were observed to be genetically stable, except one of the plant lines which segregated.
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