Growth and lipid accumulation of Rhodotorula marina IFO 0879 by fermentation of lactose
1988
Kim, Y.S. | Yu, T.J. (Korea Univ., Seoul (Korea R.). Dept. of Food Technology)
The optimum conditions for growth and lipid production of Rhodotorula marina IFO 0879 were investigated. The optimum temperature and pH of cultivation was 30deg C and pH 6.0-7.0, respectively. During shaking of the culture for 8days at 30deg C, the maximum cell biomass of Rh. marina was 9.82g per liter of the medium, and the lipid content obtained was 35.4(w/w) of the dry cell biomass. Lactose and glucose were the most effective carbon sources for the lipid production. Ammonium sulfate was found to be the most nitrogen in culture medium and the growth of the yeast was retarded, whereas its growth was favored at high concentrations with decreased lipid yield. When lactose was added during fermentation, in the initial stage cell biomass and lipid production were lower than those of the control, but in the later stage the trend were reversed. The major fatty acids of yeast lipid were palmitic acid (20.3 %), oleic acid (46.6%) and linoleic acid (16.2%)
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