Isolation of protoplasts from Flammulina velutipes
1988
Yea, U.H. | Shin, G.C. (Chungnam National Univ., Taejon (Korea R.). Coll. of Agriculture) | Yoo, Y.B. | Park, Y.H. (Rural Development Administration, Suwon (Korea R.). Agricultural Sciences Inst.)
To obtain basic information for the genetic analysis and breeding of Flammulina velutipes, some factors affecting the release of protoplasts from the fungus were studied. Potato Dextrose peptone Agar medium was suitable for the growth of the mycelium and the protoplast formation of F. velutipes. The culture age for the high yields of protoplast was 5 days on PDPA. Few protoplasts were formed from the mycelium cultured on Mushroom minimum Media. The highest yield of protoplasts was obtained in enzyme solution containing Novozyme 234 plus cellulase CP at 10 mg ml** (-1) concentration, while a half amount of protoplasts was obtained in enzyme solution containing Novozyme 234 only. The optimal reaction time of the mycelium in the lytic enzyme mixtures was 3 hours. The best osmotic stabilizer for the protoplast formation of the mycelium was 0.6M sucrose without buffer at pH 6.2
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