Isolation of Nif-mutants through transposon mutagenesis in Enterobacter agglomerans 339
1988
Min, B.W. | Lee, H.S. (Kyunghee Univ., Seoul (Korea R.). Coll. of Liberal Arts and Sciences)
Three Nif-mutants were isolated from Enterobacter agglomerans 339 through transposon mutagenesis using an RP4-mobilising system for its nif-gene characterization. All mutants had no acetylene-reduction ability. Tn5 was inserted into all conserved nif-plasmids through southern hybridization
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