Protoplast isolation and reversion from Ganoderma lucidum and Ganoderma sp.
1988
Um, S.D. | Chae, Y.A. (Seoul National Univ., Suwon (Korea R.). Coll. of Agriculture) | Yoo, Y.B. | You, C.H. | Cha, D.Y. (Rural Development Administration, Suwon (Korea R.). Agricultural Sciences Inst.)
This experiment was carried out to investigate proper conditions for protoplast isolation and reversion from Ganoderma lucidum and Ganoderma sp. In G. lucidum, 10 mg. ml** (-1) Novozyme 234 with 0.6 M sucrose was proper for protoplast isolation. The optimal reaction time of mycelium with lytic enzyme was five hrs. Protoplast isolation from four-day-old mycelium was the most effective. Protoplast isolation from four-day-old mycelium in G. sp. was optimum in the combination of Novozyme 234 and Beta-glucuronidase with 0.6 M sucrose. MCM was suitable for reversion in G. lucidum while SCM was good for G. sp. The most effective osmoticum stabilizer for protoplast reversion in G. lucidum and G. sp. was 0.6 M sucrose
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