A simple technique to obtain nodulation of chickpea and some large-seeded legumes in agar tubes
1988
Lakshminarayana, K. | Narula, N. | Tauro, P. (Haryana Agricultural Univ., Hisar (India). Dept. of Microbiology)
A simple technique adapted to trap numerous air bubbles in agar deeps during their preparation was found to help nodulation in chickpea, soyabean, garden pea and other large-seeded legumes. Nodulation was consistently high in tubes of various sizes. Nodulation of chickpea and of soybean occurred at 2-4 % agar and 0.75-2 % agar concentration, respectively. The technique enhanced the precision of plant infection tests for enumerating chickpea rhizobia in soils. Nitrogen fixing activities of nodulated soyabean plants could also be determined
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