Micropropagation of Musa AAA cv. Poyo in the Ivory Coast
1988
Mateille, T. | Foncelle, B. (ORSTOM, Abidjan (Cote d'Ivoire))
An improved micropropagation method for the Poyo banana clone is described in which 4 concentrations of benzyladenine (BA) were used: 4.5 m m, 9.0 m m, 22.5 m m and 45 m m. Main apices of sucker buds and lateral buds are stimulated by a high concentration of benzyladenine in the absence of auxin. Proliferation was best achieved with the 22.5 m m concentration, reaching a multiplication rate of 2.8 every 3 weeks. After transplantation, shoot proliferation is achieved with the same concentration of cytokinin. Shoot elongation and rooting are obtained simultaneously on a medium containing no phytohormone and 10 g/l sucrose. Rooting is improved when the bottoms of the culture tubes with the medium are set in darkness; rooting is then maximal at 97.9 %
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