In-vitro culture of Dioscorea rotundata embryos
1984
Okezie, C.E.A. | Nwoke, F.I.O. | Okonkwo, S.N.C. (University of Nigeria, Nsukka (Nigeria). Dept of Botany)
Dioscorea rotundata (Obiaoturugo variety) embryos were found to germinate faster in Murashige/Skoog (1962) and Linsmaier/Skoog (1965) media than if left in intact seeds. The ability of the embryos to germinate in the two media increased with the time from seed harvest, an indication that recovery of seeds from dormancy is gradual and that the embryos are immature at the time of seed harvest. The latter is further supported by the embryo's gradual increase in size and transformation from globular to fan shaped during the 3-4 month dormancy. The technique used in this study could enhance breeding programs.
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