Biosynthesis of storage proteins in developing endosperm of rice (Oryza sativa L. Tribe indica cv. Nazirshail) [of Bangladesh]
1991
Sarker, S.C.
An indigenous variety of rice, Nazirshail was grown hydroponically during the transplant aman season of 1989. Single stem (uniculm) was maintained. Total protein contents of the endosperms were analysed from 2 days after flowering (DAF) up to maturity of the grain. Synthesis of storage protein was found to be started from 4 DAF in the developing endosperms. Sodium-dodycil-sulfate-polyacrylamide-gel-electrophoresis (SDS-PAGE) revealed that glutelin and prolamin started to be synthesized at around 4 DAF and 9 DAF, respectively. Glutelin was initially synthesized as a large molecular weight precursor polypeptide which subsequently cleaved into two subunit structures of mature glutelin. Maximum content of this precursor protein was found to be at around 10 DAF. Cleavage into glutelin subunits continued, maintaining the steady state concentration of the precursor up to maturity of the grain. The molecular weights of the precursor and the two subunits of glutelin were determined to be 57 kDa, 37-39 kDa and 21-22 kDa, respectively.
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