Passage of ova and embryos in cows superovulated with serum gonadotropin
1992
Holy, L. | Vanatka, F. | Lopatarova, M. (Vysoka Skola Veterinarni, Brno (CSFR))
The passage and localization of eggs and embryos in individual segments of the reproductive tract of superovulated cows were studied in this work. For the induction of superovulation, serum gonadotropin at a dose of 2500-3500 I.U. was used, in combination with 0.5 mg of Cloprostenol. The start of superovulation fell on days 9 to 12 of the sexual cycle and was conditioned by the presence of the corpus luteum (CL). After the onset of the heat, 2-3 inseminations were carried out using fresh semen. Donor cows were slaughtered 3, 4, 5, 6, and 7 days after the second insemination and isolated reproductive organs were divided into five segments (two on oviducts and three on uterine horns) by the applied ligature. In laboratory conditions superovulation response was determined accurately, the volume of ovaries was assessed according to water displacement and the segments of oviducts and uterus were rinsed. 3, 4, 5, 6, and 7 days after insemination 18.1 (+-3.55), 12.4 (+-0.91), 19.2 (+-2.86), 20 and 23 (+-2.44) CL on average were recorded, which corresponded to the ovulation of 64.50, 56.71 and 72 % of stimulated follicles. Within 3 to 7 days after insemination nearly triple enlargement of ovaries was also observed. During the lavage of individual segments of the tubular reproductive tract, 38 % of eggs and embryos were detected in the uterus as early as 3 days after the stage of insemination. Four days after insemination, about 64 % of eggs and embryos were found in the uterus. On day 5 after insemination, 92 % of eggs and embryos were released into the uterus. In 6 days after insemination still 1.6 per cent of embryos were flushed out of the oviduct. Free blastocysts, however, were not recorded at that time.
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