Tannin contents of Thai peanut cultivars and their effects on growth inhibition of Aspergillus flavus
1988
Chintana Oupadissakoon (Kasetsart Univ., Bangkok (Thailand). Faculty of Agro-Industry. Dept. of Product Development)
Tannin concentrations in seed coat of 11 peanut cultivars from Kasetsart University peanut breeding program were determined. These peanut cultivars were RCM-387, Shulamit, NC 2, Tainan 9, KUP-080, KUP-421, KUP-448, KUP-476, KUP-507, KUP-573 and KUP-615. Tannin was extracted from seed coat by boiling one gram of seed coat in 400 ml. distilled water for 30 minutes, then making up the volume to 500 ml. Folin-Denis reagent method was used in measuring the color that developed at 760 nm. in Spectronic 21. The effect of seed coat tannins on the inhibition of Aspergillus parasiticus growth was determined by using methanol extracted, watersoluble material from peanut seed coats. Water solution of known concentration was prepared, autoclaved, and 0.3 ml was mixed in the plate with Potato Dextrose Agar. The 0.5 mm. plug of PDA from an actively growing Aspergillus parasiticus was placed in the center of the plate. Radicle growth from the plug was measured after 5 hr at 25 deg. Celsius. Percent inhibition was calculated. The peanut cultivar KUP-615 was found to have the highest amounth of tannin (19.53 %), followed by NC 2 (17.54 %) and Shulamit (16.97 %). These three cultivars also showed the highest rate of fungus inhibition. KUP-476 showed the lowest tannin content (6.23 %) and the porest rate of fungus inhibition.
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