Alcohol dehydrogenase isoenzymes from Nicotiana tabacum include ADH of both N. sylvestris and N. tomentosiformis
1994
Zarsky, V. | Chomatova, S. | Tupy, J. (Akademie Ved Ceske Republiky, Prague (Czech Republic). Ustav Experimentalni Botaniky)
Electrophoretic separation of seed alcohol dehydrogenase (ADH) from Nicotiana tabacum on 12 % starch gels at pH 7.8 produced only one band with an apparent Rf of 0.65, which confirmed earlier reports. The same was found with pollen ADH. However, in polyacrylamide gel isoelectric focusing, seed ADH separated into three distinct bands with apparent pI of 5.33, 5.42 and 5.50. The pI 5.33 isoenzyme was found to be the essential from in N. sylvestris seeds. The analysis of charge properties of N. tomentosiformis seed ADH showed only one isoenzyme with pl of 5.56. These results present further evidence that N. tabacum has arisen from a cross between a N. sylvestris predecessor and an ancestral type of N. tomentosiformis. The presence of the pI 5.42 from in N. tabacum is consistent with reported formation of heterodimeric ADH in tobacco hybrids.
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